• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination. • Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis. • Efficient: >90% mut
• Mutation sites on both primers to improve mutation efficiency. • Partially overlapping primers for exponential DNA amplification. • Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR Su
•5 minutes fast ligation of Pfu-amplified PCR products. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable for larger fragment cloning. •KAN and AMP resistance genes for selection.
•5 minutes fast ligation of Taq-amplified PCR products. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable for short and large fragment cloning. •Kanamycin and Ampicillin resistance genes for selection.
•5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing.
•5 minutes fast ligation of Taq-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing.
•Fast: 15 minutes. •Broad: no restriction enzyme digestions. Can be cloned into any sites. •High efficiency: up to 95% cloning efficiency. •Seamless: no extra sequences introduced; up to 5 fragments assembly.